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Fluorescence Lifetime Measurement

Fluorescence lifetime measurements based on near field or confocal microscopy find applications in various fields such as biochemistry and analytical chemistry, medicine, pharmacology, photophysics and photochemistry, and environmental research.

Over the past few years we have witnessed a tremendous surge of interest in single molecule detection (SMD) by Time Correlated Single Photon Counting technique (TCSPC). This application note explains the advantages brought by the visible single photon counters over commercially available photomultiplier tubes (PMTs) and single photon counting modules for fluorescence lifetime measurements.